Determination of Dye Concentration Using a UV-Vis Spectrophotometer

Location:

CL 1 Dalton Lab

Instructor:

Yunjuan, Zhang

Instructions:

In this experiment, you will determine the concentration of Allura Red Dye (FD&C Red No. 40) in a drink. By mixing a series of known concentration solutions of the dye and measuring absorbance ultraviolet-visible (UV-Vis) light for each, a calibration curve can be made to determine the unknown drink concentration.

Equipment:

UV-Vis Spectrophotometer

Manual

Determination of Dye Concentration Using a UV-Vis Spectrophotometer