Genotyping on ALDH2 using PCR
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of PCR and using allele-specific PCR (ASPCR) to detect the genotype of ALDH2 gene (ALDH2*1 or ALDH2*2). Equipment: Thermal cycler, Horizontal electrophoresis systems, Gel image analysis system Lab Manual: Genotyping on ALDH2 using PCR
Exploring the Microscopic World
Location: Darwin Lab Instructor: Qi ZHANG Instructions: You'll observe a variety of specimens on prepared slides under the upright microscopes. Get ready to explore a world that's too small to see with just your eyes! Equipment: Upright microscope
Extracting DNA from bananas
Location: Li Shizhen Lab Instructor: Qi ZHANG Instructions: In this experiment, you will extract and observe DNA from bananas. Equipment:
Basic microscopy
Location: Darwin Lab Instructor: Qi ZHANG Instructions: Understand the basic principles and structure of the upright microscope and the inverted fluorescence microscope. Be able to select appropriate microscope based on one's own experimental requirements. Observe and identify the differences between various samples under magnification. Learn the fluorescent staining techniques of live cell samples. Correctly use and handle a microscope, take photo, and analyze and interpret imaging data. Equipment: Upright microscope, Inverted fluorescence microscope
DNA extraction
Location: Li Shizhen Lab Instructor: Qi ZHANG Instructions: Learning the basic methods and principles of bacterial plasmid DNA extraction and quality measurement, restriction enzymes digestion, and detection by agarose gel electrophoresis. Equipment: Centrifuge, Nanodrop One spectrophotometer, Horizontal electrophoresis systems, Gel image analysis system
Determination of Chemical Oxygen Demand (COD) in Water (Potassium Permanganate Method)
Location: CL 2 Mendeleev Lab Instructor: Yunjuan, Zhang Instructions: The level of Chemical Oxygen Demand (COD) in water is one of the important indicators of water quality pollution. It is a parameter that is frequently measured in environmental protection and water quality control. COD refers to the milligrams of oxygen consumed when reducing substances in 1 liter of water are oxidized under specific conditions. Equipment: None
Analysis of Structure of Sodium Chloride and Polystyrene Using X-Ray Diffration
Location: CL 1 Dalton Lab Instructor: Yunjuan, Zhang Instructions: In this experiment we will record the diffraction peaks of an ionic solid (sodium chloride) and an organic polymer (polystyrene) by X-Ray Diffraction (XRD). Analysis of the data provides insight to the nature of the compounds themselves. Equipment: XRD Manual Analysis of Structure of Sodium Chloride and Polystyrene Using X-Ray Diffration
Determination of Relative Molecular Mass of Glucose by Freezing Point Depression Method
Location: CL 1 Dalton Lab Instructor: Yunjuan, Zhang Instructions: When a solute is dissolved in a solvent, the freezing point of the solvent decreases, which is one of the colligative properties of dilute solutions. The depression of the freezing point of a non-volatile and non-electrolyte dilute solution is directly proportional to the molal concentration of the solution. Equipment: Self-cooling Freezing Point Tester
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