Linear Sweep Voltammetry
Location: CL2 Mendeleev Lab Instructor: Yunjuan, Zhang Instructions: Master the principles of linear sweep voltammetry. Master the use and maintenance of electrochemical analysis systems. Master the basic principles of quantitative analysis using linear sweep voltammetry. Equipment: None Manual Linear Sweep Voltammetry
Preparation and Properties of Buffer Solutions
Location: CL2 Mendeleev Lab Instructor: Yunjuan, Zhang Instructions: Master the principles and methods of preparing buffer solutions. Deepen the understanding of the properties of buffer solutions. Master the use of pipettes and variable volume pipette. Master the use of a pH meter. Equipment: None Manual Preparation and Properties of Buffer Solutions
Protein separation using SDS-PAGE
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of SDS-PAGE and running protein samples in handcast polyacrylamide gel Equipment: Mini-PROTEAN Tetra cell electrophoresis system, Trans-Blot Turbo transfer system Lab Manual: Protein separation using SDS-PAGE
Protein detection using western blot
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of western blot and performing an immunodetection of beta-actin. Equipment: Rocker shaker, ChemiDoc Imaging System Lab Manual: Protein detection using western blot
Genotyping on ALDH2 using PCR
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of PCR and using allele-specific PCR (ASPCR) to detect the genotype of ALDH2 gene (ALDH2*1 or ALDH2*2). Equipment: Thermal cycler, Horizontal electrophoresis systems, Gel image analysis system Lab Manual: Genotyping on ALDH2 using PCR