Protein separation using SDS-PAGE
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of SDS-PAGE and running protein samples in handcast polyacrylamide gel Equipment: Mini-PROTEAN Tetra cell electrophoresis system, Trans-Blot Turbo transfer system Lab Manual: Protein separation using SDS-PAGE
Protein detection using western blot
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of western blot and performing an immunodetection of beta-actin. Equipment: Rocker shaker, ChemiDoc Imaging System Lab Manual: Protein detection using western blot
Genotyping on ALDH2 using PCR
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of PCR and using allele-specific PCR (ASPCR) to detect the genotype of ALDH2 gene (ALDH2*1 or ALDH2*2). Equipment: Thermal cycler, Horizontal electrophoresis systems, Gel image analysis system Lab Manual: Genotyping on ALDH2 using PCR