Protein separation using SDS-PAGE
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of SDS-PAGE and running protein samples in handcast polyacrylamide gel Equipment: Mini-PROTEAN Tetra cell electrophoresis system, Trans-Blot Turbo transfer system Lab Manual: Protein separation using SDS-PAGE
Protein detection using western blot
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of western blot and performing an immunodetection of beta-actin. Equipment: Rocker shaker, ChemiDoc Imaging System Lab Manual: Protein detection using western blot
Genotyping on ALDH2 using PCR
Location: Li Shizhen Lab Instructor: Junyu XU, Qi ZHANG Instructions: Learning the basic principle of PCR and using allele-specific PCR (ASPCR) to detect the genotype of ALDH2 gene (ALDH2*1 or ALDH2*2). Equipment: Thermal cycler, Horizontal electrophoresis systems, Gel image analysis system Lab Manual: Genotyping on ALDH2 using PCR
Exploring the Microscopic World
Location: Darwin Lab Instructor: Qi ZHANG Instructions: You'll observe a variety of specimens on prepared slides under the upright microscopes. Get ready to explore a world that's too small to see with just your eyes! Equipment: Upright microscope
Extracting DNA from bananas
Location: Li Shizhen Lab Instructor: Qi ZHANG Instructions: In this experiment, you will extract and observe DNA from bananas. Equipment:
Basic microscopy
Location: Darwin Lab Instructor: Qi ZHANG Instructions: Understand the basic principles and structure of the upright microscope and the inverted fluorescence microscope. Be able to select appropriate microscope based on one's own experimental requirements. Observe and identify the differences between various samples under magnification. Learn the fluorescent staining techniques of live cell samples. Correctly use and handle a microscope, take photo, and analyze and interpret imaging data. Equipment: Upright microscope, Inverted fluorescence microscope
DNA extraction
Location: Li Shizhen Lab Instructor: Qi ZHANG Instructions: Learning the basic methods and principles of bacterial plasmid DNA extraction and quality measurement, restriction enzymes digestion, and detection by agarose gel electrophoresis. Equipment: Centrifuge, Nanodrop One spectrophotometer, Horizontal electrophoresis systems, Gel image analysis system